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系所資訊
李易展 教授

李易展 教授

美國羅徹斯特大學 分子病理暨實驗室醫學博士

專長:分子細胞生物、放射生物、放射腫瘤
辦公室: 實驗大樓 B318室
電話:(02)2826-7189(office) (02)2826-7000 #5570(lab)
電子信箱:yjlee2@ym.edu.tw
諮詢時間:週二 10:00 ~ 12:00
 
 
 

研究領域

研究領域-李易展 教授

 

研究興趣

  癌症是威脅人類生命的主要疾病之一。對癌細胞發生的成因已有諸多的報導,但對癌症的治療仍在尋求最有效的方法。臨床上放射治療仍是常用的方法之一,但對已經有轉移現象 (Metastasis)的腫瘤則缺乏顯著的效果。因此,若能尋求適當的因子其可增加放射治療的效果 (如增加輻射敏感度) 並同時抑制腫瘤的轉移,相信可對癌症治療提供新的治療途徑。
  肌動蛋白骨架 (Actin Cytoskeleton) 是癌細胞轉移重要的生理組成物之一。小 GTPase Rho 家族蛋白質 (small GTPase Rho protein family),包括Rho,Rac 及 Cdc42,可接受胞外訊息的刺激促進細胞生長,同時形成肌動蛋白細胞骨架供細胞附著,伸展,運動,甚至生長。目前已知有30%的癌細胞與此類蛋白的異變有關。Rho 等蛋白可激活磷酸酶 ROCK 或是 PAK等,使其活化 LIM kinase (1)。LIM kinase 在細胞內唯一已知的基質為Cofilin, 是肌動蛋白去聚合因子家族蛋白的一員 (Actin Depolymerization Factor ),約有21kD的大小,主要功能是加速actin dynamics,幫助細胞的移動及化驅性,而LIM kinase 的功能即在於磷酸化並調節 cofilin 活性 (2)。到目前為止,cofilin 是否直接影響癌細胞生長及入侵轉移仍不清楚。而 Rho-LIM kinase的訊息傳導是否僅透過 cofilin 的活性調節達到細胞變異的目的也需進一步探討。
  本實驗室主要研究興趣於探討cofilin 分子做為基因放射治療的可能性,透過分子影像技術於動物體內追蹤以cofilin 為主的基因轉殖細胞所形成的腫瘤,其輻射敏感度是否如體外試驗一般,且轉移入侵能力也受到抑制。另外,cofilin 與其他分子表現調節的關係也將在此計畫中探討之,包括利用microarray檢測在大量表現cofilin的細胞中其基因表現圖譜的變化。此研究結果將有助於癌症治療及新藥開發的可能性。

實驗室成員

碩士班 (入學年度)
 
  1.邱莘予-陽明大學醫放系畢業(103)
  2.杜元亨-陽明大學醫放系畢業(103)
  3.張淳湲-陽明大學醫放系畢業(102)
  4.廖曼均-陽明大學醫放系畢業(102)
  5.黃天恆-陽明大學醫放系畢業(101)
  6.林詩婷-陽明大學醫放系畢業(101)
  7.羅佳茜-陽明大學醫放系畢業(100)
  8.陳盈伶-陽明大學醫放系畢業(100) 
 
博士班 (入學年度)
 
  1.王柏勝-慈濟技術學院醫放系畢業(100)
  2.林亮廷-陽明大學醫放系畢業(99)
  3.蔡政翰-陽明大學醫放系畢業(98)
 

學術著作(2011.01-2016.01)

Publications (* as corresponding or co-corresponding author)
  1. Chun-Yuan Chang**, Jyh-Der Leu**, Yi-Jang Lee#. The actin depolymerizing factor (ADF)/cofilin signaling pathway and DNA damage responses in cancer . INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2015;16(2):4095-4120 (SCI).(Impact factor: 2.862;Rank: 29.30%)
  2. Jyh-Der Leu**, Bo-Shen Wang**, Shu-Jun Chiu**, Chung-Yun Chang**, Chien-Chih Chen**, Fu-Du Chen**, Shiirevnyamba Avirmed**, and Yi-Jang Lee#. Combination of fisetin and ionizing radiation suppresses the growth of mammalian colorectal cancers in the xenograft tumor model (In Press). Oncology Letters 2015;: (SCI).(Impact factor: 1.554;Rank: 81.99%)
  3. Guo HW**, Lin LT**, Chen PH**, Ho MH**, Huang WT**, Lee YJ**, Chiou SH**, Hsieh YS**, Dong CY**, Wang HW#. Low-fluence rate, long duration photodynamic therapy in glioma mouse model using organic light emitting diode (OLED). Photodiagnosis and Photodynamic Therapy 2015;: (SCI).(Impact factor: 2.014;Rank: 72.04%)
  4. Baasansuren S**, Po-Shen Wang**, Ulziisaikhan E**, Amarsaukhan S**, Yi-Jang Lee**, Shiirevnyamba A#. Association between MDM2-SNP309 polymorphism and bladder cancer in Mongolians. Journal of Mongolian Medical Sciences 2015;1(171):4-8 (OI).
  5.  Cheng-Han Tsai**; Liang-Ting Lin**; Chung-Yih Wang**; Yu-Wen Chiu**; Yen-Ting Chou**; Shu-Jun Chiu**; Hsin-Ell Wang**; Ren-Shyan Liu**; Chung-Yi Wu**; Pei-Chia Chan**; Muh-Hwa Yang**; Shih-Hwa Chiou**; Man-Jyun Liao**; Yi-Jang Lee#. Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA . BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 2015;1852(5):851-861 (SCI).(Impact factor: 4.882;Rank: 16.44%)
  6. Liang-Ting Lin**, Chih-Hsien Chang**, Hsiang-Lin Yu**, Ren-Shyan Liu**, Hsin-Ell Wang**, Shu-Jun Chiu**, Fu-Du Chen**, Te-Wei Lee**, Yi-Jang Lee#. Evaluation of the therapeutic and diagnostic effects of PEGylated liposomes embedded rhenium-188 on human non-small cell lung cancer using the small animal model . Journal of Nuclear Medicine 2014;55(11):1864-1870 (SCI).(Impact factor: 6.16;Rank: 3.20%)
  7. Yao-Huei Huang**, Pei-Ming Yang**, Qiu-Yu Chuah**, Yi-Jang Lee**, Yi-Fen Hsieh**, Chih-Wen Peng** and Shu-Jun Chiu#. Autophagy promotes radiation-induced senescence but inhibits bystander effects in human breast cancer cells (Accepted). Autophagy 2014;: (SCI).(Impact factor: 11.753;Rank: 8.15%) 
  8. Ying-Ling Chen**, Chung-Yih Wang**, Feng-Yi Yang**, Bo-Sheng Wang**, Jason Y. Chen**, Liang-Ting Lin**, Jyh-Der Leu**, Su-Jun Chiu**, Fu-Du Chen**, Yi-Jang Lee# and Wei R. Chen#. Synergistic effects of the immunoadjuvant glycated chitosan with high-intensity focused ultrasound on suppression of metastases in a syngeneic breast tumor model . Cell Death & Disease 2014;: (SCI).(Impact factor: 5.014;Rank: 27.17%)
  9. Shu-Jun Chiu**, En-Chi Liao**, Ying-Ting Hsu**, Qiu-Yu Chuah**, Yi-Jang Lee**, Jing-Yi Hu**, Tsui-Chin Huang**, and Pei-Ming Yang#. Radiation induces senescence and a bystander effect through metabolic alterations . Cell Death & Disease 2014;: (SCI).(Impact factor: 5.014;Rank: 27.17%)
  10.  Liang-Ting Lin**, Shih-Hwa Chiou**, Te-Wei Lee**, Ren-Shyan Liu**, Jeng-Jong Hwang**, Chih-Hsien Chang**, Hsin-I Ma**, Yi-Jang Lee#. A comparative study of primary and recurrent human glioblastoma multiforme using the small animal imaging and molecular expressive profiles . MOLECULAR IMAGING AND BIOLOGY 2013;15(3):262-272 (SCI).(Impact factor: 2.774;Rank: 26.40%)
  11. Yi-Chu Yu**, Pei-Ming Yang**, Qiu-Yu Chuah**, Yao-Huei Huang**, Chih-Wen Peng**, Yi-Jang Lee**, Shu-Jun Chiu#. Radiation-induced senescence in securin-deficient cancer cells promotes cell invasion involving the IL-6/STAT3 and PDGF-BB/PDGFR pathways (DOI: 101038/srep01675). Scientific Reports 2013;3(1675):1-11 (SCI).(Impact factor: 5.578;Rank: 8.77%)
  12. Ying-Feng Chang**, Cheng-Han Chao**, Lih-Yuan Lin**, Cheng-Han Tsai**, Chien Chou** and Yi-Jang Lee#. Determination of urine cofilin-1 level in acute kidney injury using a high-throughput fiber optic biosensor . JOURNAL OF BIOMEDICAL OPTICS 2013;19(1):011004-1-011004-6 (SCI).(Impact factor: 2.859;Rank: 14.94%)
  13.  Liang-Ting Lin**, Bo-Sheng Wang**, Jyh-Cheng Chen**, Chi-Hsien Liu**, Chien Chou**, Shu-Jun Chiu**, Wen-Yi Chang**, Ren-Shyan Liu**, C. Allen Chang**, and Yi-Jang Lee#. mPlum-IFP 14 fluorescent fusion protein may display Forster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging. JOURNAL OF BIOMEDICAL OPTICS 2013;18 (12):126013 (SCI).(Impact factor: 2.859;Rank: 14.94%)
  14. Wei-Hsun Wang**, I-Tsang Chiang**, Yu-Chang Liu**, Fei-Ting Hsu**, Hong-Wen Chen**, Chuan-Lin Chen**, Yi-Jang Lee**, Wuu-Jyh Lin** and Jeng-Jong Hwang#. Simultaneous Imaging of Temporal Changes of NF-κB Activity and Viable Tumor Cells in Huh7/NF-κB-tk-luc2/rfp Tumor-bearing Mice . In Vivo 2013;27(3):339-350 (SCI).(Impact factor: 0.974;Rank: 86.18%)
  15.  Jyh-Der Leu**, Yu-Wen Chiu**, Chia-Chien Lo**, Pei-Hsun Chiang**, Su-Jun Chiu**, Cheng-Han Tsai**, Jeng-Jong Hwang**, Ran-Chou Chen**, Vera Gorbunova**, Yi-Jang Lee#. Enhanced cellular radiosensitivity induced by cofilin-1 over-expression is associated with reduced DNA repair capacity . INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 2013;89(6):433-444 (SCI).(Impact factor: 1.687;Rank: 11.76%)
  16. Cheng-Han Chao**, Ying-Feng Chang**, Hsien-Ching Chen**, Lih-Yuan Lin**, Pei-Chun Yu**, Yu-Sun Chang**, Yi-Jang Lee# and Chien Chou#. Detection of urine cofilin-1 from patients hospitalized in the intensive care unit using the metal-enhanced fluorescence technique . SENSORS AND ACTUATORS B-CHEMICAL 2012;173:184-190 (SCI).(Impact factor: 4.097;Rank: 5.36%)
  17.  Li-hsin Chen**, Pei-ming Chu**, Yi-jang Lee**, Pang-hsien Tu**, Chin-wen Chi**, Hsin-chen Lee**, Shih-hwa Chiou#. Targeting protective autophagy exacerbates UV-triggered apoptotic cell death (Accepted). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2012;13(1):1209-24 (SCI).(Impact factor: 2.862;Rank: 29.30%)
  18. Shanshan Shi**, Tianfang Li**, Jonathan Holz**, Yi-Jang Lee**, Tzong-jen Sheu**, Tao Xiao**, Meng Mi#. TIMP2 Deficient Mice Develop Accelerated Osteoarthritis via Promotion of Angiogenesis upon Destabilization of the Medial Meniscus (Accepted). BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2012;423(2):366-372 (SCI).(Impact factor: 2.297;Rank: 56.16%)
  19. Ying-Ling Chen**, Shiau-Yi Wang**, Ren-Shyan Liu**, Hsin-Ell Wang**, Jyh-Cheng Chen**, Shih-Hwa Chiou**, C. Allen Chang**, Liang-Ting Lin**,David Tan Tap Wei** and Yi-Jang Lee#. Remnant living cells that escape cell loss in late-stage tumors exhibit cancer stem cells-like characteristics (Accepted). Cell Death & Disease 2012;3:e399 (SCI).(Impact factor: 5.014;Rank: 27.17%)
  20. Cheng-Han Tsai** and Yi-Jang Lee#. Focus on ADF/cofilin: beyond actin cytoskeletal regulation (Accepted). ISRN Cell Biology 2011;2012:1-7 (OI).
  21. Jyh-Der Leu**, Chung-Yih Wang**, Han-Yi Tsa**i, I-Feng Lin**, Ran-Chou Chen** and Yi-Jang Lee#. Involvement of p53 R72P polymorphismin the association of MDM2-SNP309 with breast cancer . ONCOLOGY REPORTS 2011;25:1755-1763 (SCI).(Impact factor: 2.301;Rank: 65.40%)第一或通訊作者
  22.  Pei-Ming Chu**, Shih-Hwa Chiou**, Tsann-Long Su**, Yi-Jang Lee**, Li-Hsin Chen**, Yi-Wei Chen**, Sang-Hue Yen**, Ming-Teh Chen**, Ming-Hsiung Chen**, Yang-Hsin Shih**, Pang-Hsien Tu** and Hsin-I Ma#. Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response . Radiation Oncology 2011;6(7):1-13 (SCI).(Impact factor: 2.546;Rank: 32.00%)
  23. Shin-Yi Liu, Yi-Jang Lee#, Te-Chang Lee**. Association of platelet-derived growth factor receptor accumulation with increased oxidative stress and cellular injury in sestrin 2 silenced human glioblastoma cells. FEBS LETTERS 2011;585(12):1853-1858 (SCI).(Impact factor: 3.169;Rank: 34.25%)
  24. Ying-Feng Chang**, Li-Chen Su#, Yi-Jang Lee**, Ying-Chang Li**, Yi-Tsen Lu**, Jo-Ping Hsieh**, Ja-An Annie Ho**, Chii-Chang Chen**, Cheng-Chung Lee**, Chien Chou**. Discrimination of Breast Cancer by Measuring Prostate-Specific Antigen Levels in Women's Serum. ANALYTICAL CHEMISTRY 2011;83(13):5324-5328 (SCI).(Impact factor: 5.636;Rank: 5.41%)
  25.  Tien-Heng Huang**, Shu-Jun Chiu**, Pei-Hsiuang Chiang**, Wen-Tai Li**, Shih-Hwa Chiou**, and Chiung-Tong Chen**, Yi-Jang Lee#. Anti-proliferative effects of N-heterocyclic indolyl glyoxylamides derivatives on human lung cancer cells . ANTICANCER RESEARCH 2011;31:3407-3416 (SCI).(Impact factor: 1.826;Rank: 76.78%) 
     
Invited review article and book chapter
  1. Yi-Jang Lee, Pei-Hsun Chaing and Peter C. Keng. Cyotskeletal organization and Rb tumor suppressor gene (2011). Retinoblastoma, ISBN 978-953-307-845-8. (In Press)

Patent
  1. Liao GJ, Lee YJ, Lee YH, Chen LL, Chu WS. Rhodosporidium D-amino acid oxidase. USA, Taiwan, and New Zealand.
  2. Yi-Jang Lee and Cheng-Han Tsai. Method for evaluating cell aging by expression level of cofilin. USA
  3. Shiau-Yi Wang and Yi-Jang Lee. Methods for In vivo Identification of Cancer Initiating Cells by Multimodality Reporter Gene Imaging. USA
  4. Yi-Jang Lee and Liang-Ting Lin, Bo-Sheng Wang. 螢光融合蛋白. 中華民國.